Instead, on N-PK51, the emission detected in the far-red under red excitation was 2.4 times higher compared to D263M, more than compensating the small improvement in the far-red emission upon blue excitation in a hypothetical 488-633 couple for simultaneous two-color applications. We did not find a material significantly improving the low SNRs for Abberior Star 635p in two-color configurations shown in Figure 1D; therefore, in the following we will mostly show results on dyes with excitations at 488 nm and 561 nm.
《Optimized two-color single-molecule tracking of fast-diffusing membrane receptors》
2023.3.20
Verse 1:
Abberior STAR 635p, a dye that’s far-red,
With 635nm light, the SNR’s dead,
In MatTek, Fluorodish, and N-Pk51,
The background’s high, TIRF’s not fine.
Chorus:
Abberior STAR 635p, can’t get it right,
Low SNR, it’s just not tight,
Trying to detect signals, such a plight,
Far-red dye, it’s a tough fight.
Verse 2:
Two-color configurations, we gave it a try,
Abberior STAR 635p, no hope to fly,
We searched for a material, to no avail,
488nm and 561nm, let’s set sail.
Chorus:
Abberior STAR 635p, can’t get it right,
Low SNR, it’s just not tight,
Trying to detect signals, such a plight,
Far-red dye, it’s a tough fight.
Verse 3:
TIRF background, it’s quite a mess,
Abberior STAR 635p, under duress,
Let’s find a new dye, with more finesse,
And light up TIRF, with success.
Chorus:
Abberior STAR 635p, can’t get it right,
Low SNR, it’s just not tight,
Trying to detect signals, such a plight,
Far-red dye, it’s a tough fight.
